Anticorps Monoclonal anti-ICT1

ICT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F11E6

N° de cat : 68898-3-Ig

Synonymes

1F11E6, 39S ribosomal protein L58, mitochondrial, DS1, DS-1, EC:3.1.1.29

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Galerie de données de validation

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  WB analysis using 68898-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68898-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW480 using 68898-3-Ig

  SW480 cells were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 68898-3-Ig

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ICT1 antibody (68898-3-Ig, Clone: 1F11E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HEK-293, cellules HeLa, cellules LNCaP, cellules SW480
• Résultats positifs en IF/ICC: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68898-3-Ig cible ICT1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ICT1 Protéine recombinante Ag33109
• Nom complet: immature colon carcinoma transcript 1
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 21-24 kDa
• Numéro d’acquisition GenBank: BC015335
• Symbole du gène: ICT1
• Identification du gène (NCBI): 3396
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells.

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