Anticorps Monoclonal anti-ICT1

ICT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F11E6

N° de cat : 68898-3-PBS

Synonymes

1F11E6, 39S ribosomal protein L58, mitochondrial, DS1, DS-1, EC:3.1.1.29

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Galerie de données de validation

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  WB analysis using 68898-3-Ig (same clone as 68898-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 68898-3-Ig (same clone as 68898-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

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  WB analysis of SW480 using 68898-3-Ig (same clone as 68898-3-PBS)

  SW480 cells were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 68898-3-Ig (same clone as 68898-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ICT1 antibody (68898-3-Ig, Clone: 1F11E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68898-3-PBS in a different storage buffer formulation.

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Informations sur le produit

68898-3-PBS cible ICT1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ICT1 Protéine recombinante Ag33109
• Nom complet: immature colon carcinoma transcript 1
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 21-24 kDa
• Numéro d’acquisition GenBank: BC015335
• Symbole du gène: ICT1
• Identification du gène (NCBI): 3396
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells.

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