Anticorps Monoclonal anti-ID1

ID1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1H3G11

N° de cat : 67827-1-PBS

Synonymes

1H3G11, bHLHb24, Class B basic helix-loop-helix protein 24, DNA-binding protein inhibitor ID-1, ID

---

Galerie de données de validation

• 

  WB analysis using 67827-1-Ig (same clone as 67827-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67827-1-Ig (ID1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67827-1-Ig (same clone as 67827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67827-1-Ig (ID1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67827-1-Ig (same clone as 67827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67827-1-Ig (ID1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67827-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67827-1-PBS cible ID1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ID1 Protéine recombinante Ag13359
• Nom complet: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC000613
• Symbole du gène: ID1
• Identification du gène (NCBI): 3397
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 18 kDa.

{{ptg:RelatedPrimaryAntibodies}}