Anticorps Polyclonal de lapin anti-ID3

• Phare
• Validé par KD/KO

ID3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10389-1-AP

Synonymes

1R21, bHLHb25, HEIR 1, HEIR1, ID3, Inhibitor of DNA binding 3

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Galerie de données de validation

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  WB analysis of Transfected HEK-293 using 10389-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10389-1-AP (ID3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10389-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10389-1-AP (ID3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293 transfectées

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

10389-1-AP cible ID3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, vison
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ID3 Protéine recombinante Ag0588
• Nom complet: inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
• Masse moléculaire calculée: 13 aa, 5 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC003107
• Symbole du gène: ID3
• Identification du gène (NCBI): 3399
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The helix-loop-helix (HLH) family of transcription factors plays a central role in the regulation of cell growth, differentiation and tumourigenesis. Members of the Id (inhibitor of DNA binding) class of these nuclear proteins are able to heterodimerise with and thereby antagonise the functions of other transcription factors of this family. Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein (ID3, synonym: HEIR-1) is a member of the ID family of HLH proteins, which lacks a basic DNA-binding domain and inhibits transcription through formation of nonfunctional dimers that are incapable of binding to DNA.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Bcl-3 regulates TGFβ signaling by stabilizing Smad3 during breast cancer pulmonary metastasis.; Authors - Xi Chen
mouse	WB	Biomedicines; BMP4 Exerts Anti-Neurogenic Effect via Inducing Id3 during Aging.; Authors - Tingting Li
rat	IHC	Cancer Sci; Cellular distributions of molecules with altered expression specific to thyroid proliferative lesions developing in a rat thyroid carcinogenesis model.; Authors - Woo Gye-Hyeong GH
mink	WB	Life Sci; Pantothenic acid promotes dermal papilla cell proliferation in hair follicles of American minks via inhibitor of DNA Binding 3/Notch signaling pathway.; Authors - Zhuo Wang ; KD Validated