Anticorps Polyclonal de lapin anti-ID4

ID4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21803-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 21803-1-AP (same clone as 21803-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21803-1-AP (ID4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21803-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 21803-1-AP (same clone as 21803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21803-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 21803-1-AP (same clone as 21803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 21803-1-AP (same clone as 21803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 21803-1-AP (same clone as 21803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21803-1-AP (ID4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21803-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21803-1-PBS cible ID4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ID4 Protéine recombinante Ag16883
• Nom complet: inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
• Masse moléculaire calculée: 161 aa, 17 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC014941
• Symbole du gène: ID4
• Identification du gène (NCBI): 3400
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ID4 (inhibitor of DNA binding 4) negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. (PMID: 19783986). ID4 levels dictate the stem cell state in mouse spermatogonia (PMID: 28087628). It is involved in the regulation of many cellular processes during both prenatal development and tumorigenesis. ID4 can be detected 25-30 kDa (PMID: 34108007, PMID: 31847356).

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