Anticorps Polyclonal de lapin anti-IDE

• Phare
• Validé par KD/KO

IDE Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21728-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 21728-1-AP (same clone as 21728-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21728-1-AP (same clone as 21728-1-PBS)

  WB result of IDE antibody (21728-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 21728-1-AP (same clone as 21728-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A2780 using 21728-1-AP (same clone as 21728-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 21728-1-AP (same clone as 21728-1-PBS)

  IP result of anti-IDE (IP:21728-1-AP, 4ug; Detection:21728-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

21728-1-PBS cible IDE dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IDE Protéine recombinante Ag16392
• Nom complet: Abeta degrading protease
• Masse moléculaire calculée: 1019 aa, 118 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC096336
• Symbole du gène: IDE
• Identification du gène (NCBI): 3416
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IDE (INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531). This antibody is specific to IDE.

{{ptg:RelatedPrimaryAntibodies}}