Anticorps Monoclonal anti-IDE

• Phare
• Validé par KD/KO

IDE Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E10G1

N° de cat : 67106-1-PBS

Synonymes

Insulin-degrading enzyme, Insulinase, Insulin protease, EC:3.4.24.56, Abeta-degrading protease

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Galerie de données de validation

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  WB analysis using 67106-1-Ig (same clone as 67106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67106-1-Ig (same clone as 67106-1-PBS)

  WB result of IDE antibody (67106-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67106-1-Ig (same clone as 67106-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 67106-1-Ig (same clone as 67106-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67106-1-Ig (same clone as 67106-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67106-1-Ig (same clone as 67106-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67106-1-Ig (same clone as 67106-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67106-1-Ig (same clone as 67106-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 67106-1-Ig (same clone as 67106-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67106-1-Ig (same clone as 67106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67106-1-Ig (same clone as 67106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67106-1-Ig (IDE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67106-1-Ig (same clone as 67106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67106-1-Ig (IDE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67106-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67106-1-PBS cible IDE dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IDE Protéine recombinante Ag17161
• Nom complet: Abeta degrading protease
• Masse moléculaire calculée: 1019 aa, 118 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC096336
• Symbole du gène: IDE
• Identification du gène (NCBI): 3416
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IDE(INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531).

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