Anticorps Monoclonal anti-IDH1

IDH1 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2A6A2

N° de cat : 66197-1-Ig

Synonymes

IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD

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Galerie de données de validation

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  WB analysis of HepG2 using 66197-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 66197-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66197-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66197-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66197-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 66197-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 66197-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 66197-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66197-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66197-1-Ig (IDH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66197-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66197-1-Ig(IDH1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules DU 145, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu pancréatique humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66197-1-Ig cible IDH1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IDH1 Protéine recombinante Ag19293
• Nom complet: isocitrate dehydrogenase 1 (NADP+), soluble
• Masse moléculaire calculée: 414 aa, 47 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC012846
• Symbole du gène: IDH1
• Identification du gène (NCBI): 3417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

Publications

Species	Application	Title
		Nat Neurosci; TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.; Authors - Florent Laferrière
human	WB	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge
human		J Immunol; Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues; Authors - Teresa W-M Fan