Anticorps Recombinant de lapin anti-IDH2

• Phare
• Validé par KD/KO

IDH2 Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B1

N° de cat : 82117-1-PBS

Synonymes

IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42

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Galerie de données de validation

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  WB analysis using 82117-1-RR (same clone as 82117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

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  WB analysis using 82117-1-RR (same clone as 82117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 82117-1-RR (same clone as 82117-1-PBS)

  WB result of IDH2 antibody (82117-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDH2 transfected HEK-293 cells. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 82117-1-RR (same clone as 82117-1-PBS)

  IP result of anti-IDH2 (IP:82117-1-RR, 4ug; Detection:82117-1-RR 1:1000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82117-1-RR (same clone as 82117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82117-1-RR (IDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82117-1-PBS cible IDH2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IDH2 Protéine recombinante Ag8779
• Nom complet: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• Masse moléculaire calculée: 452 aa, 51 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC009244
• Symbole du gène: IDH2
• Identification du gène (NCBI): 3418
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

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