Anticorps Monoclonal anti-IDH2

• Phare
• Validé par KD/KO

IDH2 Monoclonal Antibody for WB, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F10A10

N° de cat : CL488-66918

Synonymes

IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42

---

Galerie de données de validation

• 

  WB analysis using CL488-66918

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66918 (IDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using CL488-66918

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated IDH2 Monoclonal antibody (CL488-66918, Clone:1F10A10)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66918 cible IDH2 dans les applications de WB, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IDH2 Protéine recombinante Ag8950
• Nom complet: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• Masse moléculaire calculée: 452 aa, 51 kDa
• Poids moléculaire observé: 41-47 kDa
• Numéro d’acquisition GenBank: BC009244
• Symbole du gène: IDH2
• Identification du gène (NCBI): 3418
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

{{ptg:RelatedPrimaryAntibodies}}