Anticorps Polyclonal de lapin anti-IDI1

IDI1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11166-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of L02 using 11166-2-AP (same clone as 11166-2-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 11166-2-AP (same clone as 11166-2-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 11166-2-AP (same clone as 11166-2-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 11166-2-AP (same clone as 11166-2-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 11166-2-AP (same clone as 11166-2-PBS)

  IP result of anti-IDI1 (IP:11166-2-AP, 4ug; Detection:11166-2-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

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Informations sur le produit

11166-2-PBS cible IDI1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IDI1 Protéine recombinante Ag1636
• Nom complet: isopentenyl-diphosphate delta isomerase 1
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC019227
• Symbole du gène: IDI1
• Identification du gène (NCBI): 3422
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IDI1(Isopentenyl-diphosphate Delta-isomerase 1) is a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP). It belongs to the IPP isomerase type 1 family. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 26 kDa and 32 kDa.

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