Anticorps Monoclonal anti-IDO1

IDO1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

CloneNo.

3G2G11

N° de cat : 66528-1-Ig

Synonymes

CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1

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Galerie de données de validation

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  WB analysis using 66528-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66528-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 66528-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HeLa traitées à l'IFN gamma, cellules HSC-T6

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

66528-1-Ig cible IDO1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IDO1 Protéine recombinante Ag3953
• Nom complet: indoleamine 2,3-dioxygenase 1
• Masse moléculaire calculée: 403 aa, 45 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC027882
• Symbole du gène: IDO1
• Identification du gène (NCBI): 3620
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

Publications

Species	Application	Title
human	WB	Elife; PMID: 30132758; Authors - Tonya Kueck
human,mouse	WB,IHC,IF	Pharmacol Res; IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.; Authors - Xin Fang
mouse	WB	ACS Chem Neurosci; 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Induced Parkinson's Disease in Mouse: Potential Association between Neurotransmitter Disturbance and Gut Microbiota Dysbiosis.; Authors - Yuanhui Zhu
human	WB	Chin J Nat Med; Panax notoginseng saponins prevent colitis-associated colorectal cancer via inhibition IDO1 mediated immune regulation.; Authors - Xue-Ming Li
mouse	WB	Chin J Integr Med; Combination of Geniposide and Eleutheroside B Exerts Antidepressant-like Effect on Lipopolysaccharide-Induced Depression Mice Model.; Authors - Bo Zhang
mouse	WB	Mol Med Rep; Fr‑HMGB1 and ds‑HMGB1 activate the kynurenine pathway via different mechanisms in association with depressive‑like behavior.; Authors - Bo Wang