Anticorps Recombinant de lapin anti-IDO1

IDO1 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241110F2

N° de cat : 84063-3-PBS

Synonymes

241110F2, EC:1.13.11.52, IDO, IDO 1, IDO-1

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Galerie de données de validation

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  WB analysis using 84063-3-RR (same clone as 84063-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84063-3-RR (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84063-3-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84063-3-RR (same clone as 84063-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84063-3-RR (IDO1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84063-3-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84063-3-RR (same clone as 84063-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84063-3-RR (IDO1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84063-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84063-3-RR (same clone as 84063-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84063-3-RR (IDO1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84063-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84063-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84063-3-RR against Human IDO1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84063-3-PBS cible IDO1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IDO1 Protéine recombinante Ag3953
• Nom complet: indoleamine 2,3-dioxygenase 1
• Masse moléculaire calculée: 403 aa, 45 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC027882
• Symbole du gène: IDO1
• Identification du gène (NCBI): 3620
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

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