Anticorps Recombinant de lapin anti-IDS

IDS Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242054A7

N° de cat : 84623-6-PBS

Synonymes

Iduronate 2-sulfatase 42 kDa chain, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase, EC:3.1.6.13, Alpha-L-iduronate sulfate sulfatase

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Galerie de données de validation

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  WB analysis using 84623-6-RR (same clone as 84623-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84623-6-RR (IDS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84623-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84623-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84623-6-RR against Human IDS were performed. The affinity constant is 11.8 nM.

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Informations sur le produit

84623-6-PBS cible IDS dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IDS Protéine recombinante Ag31132
• Nom complet: iduronate 2-sulfatase
• Masse moléculaire calculée: 312aa,35 kDa; 550aa,62 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC006170
• Symbole du gène: IDS
• Identification du gène (NCBI): 3423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).

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