Anticorps Polyclonal de lapin anti-IFFO1

IFFO1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16041-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse brain using 16041-1-AP (same clone as 16041-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 16041-1-AP (same clone as 16041-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16041-1-AP (same clone as 16041-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 16041-1-AP (same clone as 16041-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16041-1-AP (same clone as 16041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16041-1-AP (IFFO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16041-1-AP (same clone as 16041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16041-1-AP (IFFO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16041-1-AP (same clone as 16041-1-PBS)

  Immunofluorescent analysis of Hela cells, using IFFO1 antibody 16041-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16041-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16041-1-PBS cible IFFO1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFFO1 Protéine recombinante Ag8915
• Nom complet: intermediate filament family orphan 1
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC004384
• Symbole du gène: IFFO1
• Identification du gène (NCBI): 25900
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IFFO1, also named as IFFO and Tumor antigen HOM-TES-103, is a member of the intermediate filament family which consists of primordial components of the cytoskeleton and nuclear envelope that are mainly distributed in the nucleus, followed by the cytoskeleton and cytoplasm. IFFO1 in the nucleus was identified to combine with both XRCC4 and the nuclear lamina protein lamin A/C to be involved in nonhomologous DNA end joining (NHEJ). The expression of IFFO1 is associated with tumor progression. IFFO1 could be used as a potential marker to differentiate malignant ascites cell-free tumor DNA (cftDNA) of ovarian cancer from benign controls.

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