Anticorps Recombinant de lapin anti-IFI35

IFI35 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240063C6

N° de cat : 83160-2-PBS

Synonymes

240063C6, Ifi 35, Ifi-35, IFP 35, IFP35

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Galerie de données de validation

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  IF Staining of HeLa using 83160-2-RR (same clone as 83160-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI35 antibody (83160-2-RR, Clone: 240063C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83160-2-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83160-2-RR (same clone as 83160-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83160-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83160-2-RR (same clone as 83160-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83160-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00191-2

  Cytometric bead array standard curve of MP00191-2, IFI35 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83160-2-PBS. Detection antibody: 83160-1-PBS. Standard: Ag34653. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83160-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83160-2-PBS against Human IFI35 was performed. The affinity constant is 1.93 nM.

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  Affinity and Kinetic Characterization of 83160-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83160-2-RR against Human IFI35 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83160-2-PBS cible IFI35 dans les applications de IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFI35 Protéine recombinante Ag34653
• Nom complet: interferon-induced protein 35
• Masse moléculaire calculée: 31 kDa
• Numéro d’acquisition GenBank: BC001356
• Symbole du gène: IFI35
• Identification du gène (NCBI): 3430
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IFI35 (interferon induced protein 35), also known as IFP35. It is expected to be located in cytoplasm, nucleus and extracellular space. The protein is expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Involved in several processes, including macrophage activation involved in immune response; positive regulation of defense response; and regulation of signal transduction. And the calculated molecular weight of IFI35 is 31 kDa.

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