Anticorps Recombinant de lapin anti-IFI35

IFI35 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240063C6

N° de cat : 83160-2-RR

Synonymes

240063C6, Ifi 35, Ifi-35, IFP 35, IFP35

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Galerie de données de validation

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  IF Staining of HeLa using 83160-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI35 antibody (83160-2-RR, Clone: 240063C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A549 using 83160-2-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of HeLa using 83160-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83160-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83160-2-RR against Human IFI35 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules A549, cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83160-2-RR cible IFI35 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFI35 Protéine recombinante Ag34653
• Nom complet: interferon-induced protein 35
• Masse moléculaire calculée: 31 kDa
• Numéro d’acquisition GenBank: BC001356
• Symbole du gène: IFI35
• Identification du gène (NCBI): 3430
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFI35 (interferon induced protein 35), also known as IFP35. It is expected to be located in cytoplasm, nucleus and extracellular space. The protein is expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Involved in several processes, including macrophage activation involved in immune response; positive regulation of defense response; and regulation of signal transduction. And the calculated molecular weight of IFI35 is 31 kDa.

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