Anticorps Recombinant de lapin anti-IFIT1

IFIT1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240132G11

N° de cat : 83423-1-PBS

Synonymes

IFIT 1, IFI-56K, IFI56, IFI 56K, G10P1

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Galerie de données de validation

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  WB analysis of HeLa using 83423-1-RR (same clone as 83423-1-PBS)

  IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 83423-1-RR (IFIT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 83423-1-RR (same clone as 83423-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 83423-1-RR (IFIT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR (same clone as 83423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83423-1-RR (IFIT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR (same clone as 83423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83423-1-RR (IFIT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83423-1-RR (same clone as 83423-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifit1 Recombinant Antibody (83423-1-RR, Clone:240132G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83423-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83423-1-RR against Human IFIT1 were performed. The affinity constant is 0.397 nM.

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Informations sur le produit

83423-1-PBS cible IFIT1 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFIT1 Protéine recombinante Ag19691
• Nom complet: interferon-induced protein with tetratricopeptide repeats 1
• Masse moléculaire calculée: 478 aa, 55 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC007091
• Symbole du gène: IFIT1
• Identification du gène (NCBI): 3434
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.

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