Anticorps Polyclonal de lapin anti-IFIT5

• Phare
• Validé par KD/KO

IFIT5 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 13378-1-AP

Synonymes

IFIT 5, IFIT5, RI58

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Galerie de données de validation

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  WB analysis of A375 using 13378-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13378-1-AP (IFIT5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13378-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13378-1-AP (IFIT5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 13378-1-AP

  IP result of anti-IFIT5 (IP:13378-1-AP, 4ug; Detection:13378-1-AP 1:300) with A375 cells lysate 2200ug.

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  IHC staining of human skin cancer using 13378-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13378-1-AP (IFIT5 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 13378-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13378-1-AP (IFIT5 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules PC-3
• Résultats positifs en IP: cellules A375
• Résultats positifs en IHC: tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13378-1-AP cible IFIT5 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFIT5 Protéine recombinante Ag4202
• Nom complet: interferon-induced protein with tetratricopeptide repeats 5
• Masse moléculaire calculée: 482 aa, 58 kDa
• Poids moléculaire observé: 56-58 kDa, 48 kDa
• Numéro d’acquisition GenBank: BC025786
• Symbole du gène: IFIT5
• Identification du gène (NCBI): 24138
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFIT5, also named as Interferon-induced 58 kDa protein or RI58, is a 482 amino acid protein, which contains eight TPR repeats and belongs to the IFIT family. IFIT5 localizes in the Cell projection. Interferon-induced RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs.

Publications

Species	Application	Title
human	WB	J Immunol; IFIT5 Negatively Regulates the Type I IFN Pathway by Disrupting TBK1-IKKε-IRF3 Signalosome and Degrading IRF3 and IKKε.; Authors - Na Zhang
human	WB	Cell Signal; IFIT5 positively regulates NF-κB signaling through synergizing the recruitment of IκB kinase (IKK) to TGF-β-activated kinase 1 (TAK1).; Authors - Caishang Zheng ; KD Validated
Human	WB	Acta Biochim Biophys Sin (Shanghai); IFIT5 potentiates anti-viral response through enhancing innate immune signaling pathways.; Authors - Zhang Bianhong B
human	WB,IHC	Cell Death Dis; The roles and mechanism of IFIT5 in bladder cancer epithelial-mesenchymal transition and progression.; Authors - Jun Huang
human	IHC	Oncoimmunology; Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.; Authors - Chen Zhu
human	WB	mBio; PKR Binds Enterovirus IRESs, Displaces Host Translation Factors, and Impairs Viral Translation to Enable Innate Antiviral Signaling.; Authors - Mikhail I Dobrikov

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