Anticorps Monoclonal anti-IFITM2/3

• Phare
• Validé par KD/KO

IFITM2/3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2E8D12

N° de cat : 66081-1-PBS

Synonymes

IFITM3, 1 8U, 2E8D12, DSPA2b, Interferon-induced transmembrane protein 3

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Galerie de données de validation

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  WB analysis using 66081-1-Ig (same clone as 66081-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66081-1-Ig (same clone as 66081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66081-1-Ig (same clone as 66081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66081-1-Ig (same clone as 66081-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IFITM2/3 antibody (66081-1-Ig, Clone: 2E8D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66081-1-PBS cible IFITM2/3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IFITM2/3 Protéine recombinante Ag17863
• Nom complet: interferon induced transmembrane protein 3 (1-8U)
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC070243
• Symbole du gène: IFITM3
• Identification du gène (NCBI): 10410
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.

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