Anticorps Recombinant de lapin anti-IFITM2

• Phare
• Validé par KD/KO

IFITM2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240318B6

N° de cat : 83455-5-PBS

Synonymes

1 8D, 18D, 240318B6, DSPA2c, IFITM 2

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Galerie de données de validation

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  WB analysis using 83455-5-RR (same clone as 83455-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83455-5-RR (IFITM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

  WB result of IFITM2 antibody (83455-5-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFITM2 transfected HepG2 cells. This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using IFITM2 antibody (83455-5-RR, Clone: 240318B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFITM2 antibody (83455-5-RR, Clone: 240318B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83455-5-RR (same clone as 83455-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug IFITM2 Recombinant antibody (83455-5-RR, Clone:240318B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83455-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83455-5-RR against Human IFITM2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83455-5-PBS cible IFITM2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFITM2 Protéine recombinante Ag3451
• Nom complet: interferon induced transmembrane protein 2 (1-8D)
• Masse moléculaire calculée: 132 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC009696
• Symbole du gène: IFITM2
• Identification du gène (NCBI): 10581
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in a p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also, they serve as important components of the innate immune system to restrict HIV-1 infection.

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