Anticorps Polyclonal de lapin anti-IFITM3

• Phare
• Validé par KD/KO

IFITM3 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (7)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

124

Avis (2)

N° de cat : 11714-1-AP

Synonymes

1 8U, Fragilis, IFITM3, IP15

---

Galerie de données de validation

• 

  WB analysis using 11714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11714-1-AP (IFITM3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11714-1-AP

  IP result of anti-IFITM3(IP:11714-1-AP, 4ug; Detection:11714-1-AP 1:4000) with HepG2 cells lysate 960 ug.

• 

  IF Staining of HeLa using 11714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFITM3 antibody (11714-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 11714-1-AP

  1X10^6 HeLa cells were stained with 0.2ug IFITM3 antibody (11714-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules LNCaP, cellules THP-1
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 34 publications below
• WB: See 100 publications below
• IHC: See 7 publications below
• IF: See 27 publications below
• IP: See 5 publications below
• FC: See 7 publications below
• CoIP: See 2 publications below

Informations sur le produit

11714-1-AP cible IFITM3 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, canin, Chèvre, Humain, porc, poulet, souris, Singe vert d'Afrique
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFITM3 Protéine recombinante Ag2285
• Nom complet: interferon induced transmembrane protein 3 (1-8U)
• Masse moléculaire calculée: 133 aa, 15 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC006794
• Symbole du gène: IFITM3
• Identification du gène (NCBI): 10410
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.

Publications

Species	Application	Title
human	WB	Science; A human apolipoprotein L with detergent-like activity kills intracellular pathogens.; Authors - Ryan G Gaudet
human,mouse	WB	Cell; A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling.; Authors - Noémie Alphonse
human	WB	Nature; IFITM3 restricts the morbidity and mortality associated with influenza.; Authors - Everitt AR
mouse	IHC	Science; T cell memory. Skin-resident memory CD8⁺ T cells trigger a state of tissue-wide pathogen alert.; Authors - Silvia Ariotti
human	IF	Cell Stem Cell; Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids.; Authors - Galina Popova
human	WB,IF	Cell Stem Cell; PMID: 30416070; Authors - Carolina Petrillo ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Thomas (Verified Customer) (06-29-2022)

Works fine in WB and IF

• Applications: Western Blot, Immunofluorescence

FH

Xiuqing (Verified Customer) (03-21-2022)

worked very well for western blot

• Applications: Western Blot
• Cell Tissue Type: MARC-145 cells, H1299 cells

Produits associés

IFITM2 Polyclonal antibody

12769-1-AP

IFITM1-Specific Monoclonal antibody

60074-1-Ig

IFITM2-specific Monoclonal antibody

66137-1-Ig

Osteopontin Polyclonal antibody

22952-1-AP

NAMPT/PBEF Polyclonal antibody

11776-1-AP

IFITM1 Polyclonal antibody

11727-3-AP

IFN Gamma Polyclonal antibody

15365-1-AP