Anticorps Monoclonal anti-IFITM2/3

• Phare
• Validé par KD/KO

IFITM2/3 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

14

CloneNo.

2E8D12

Avis (1)

N° de cat : 66081-1-Ig

Synonymes

1 8U, IFITM2/3, IFITM3, IP15

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Galerie de données de validation

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  WB analysis using 66081-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IHC staining of human liver cancer using 66081-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66081-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 66081-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IFITM2/3 antibody (66081-1-Ig, Clone: 2E8D12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66081-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug IFITM2/3 antibody (66081-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HeLa, cellules HL-60, cellules Jurkat, cellules K-562, cellules LNCaP, cellules MCF-7, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U2OS,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Informations sur le produit

66081-1-Ig cible IFITM2/3 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IFITM2/3 Protéine recombinante Ag17863
• Nom complet: interferon induced transmembrane protein 3 (1-8U)
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC070243
• Symbole du gène: IFITM3
• Identification du gène (NCBI): 10410
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.

Publications

Species	Application	Title
human	FC	Science; IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.; Authors - Julian Buchrieser
human	IF,FC	EMBO J; Syncytia formation by SARS-CoV-2-infected cells.; Authors - Julian Buchrieser
human	WB,IF	Proc Natl Acad Sci U S A; mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.; Authors - Guoli Shi
human	WB	EMBO Rep; Natural mutations in IFITM3 modulate post-translational regulation and toggle antiviral specificity.; Authors - Alex A Compton
human	WB	MBio; Late Endosomal/Lysosomal Cholesterol Accumulation Is a Host Cell-Protective Mechanism Inhibiting Endosomal Escape of Influenza A Virus.; Authors - Alexander Kühnl
human	WB,IF	Front Immunol; Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.; Authors - Bei Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Claudia (Verified Customer) (06-20-2022)

The antibody worked very well for the analysis of IFITM2/3 expression in permanent cell lines.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200 (immunofluorescence), 1:5000 (Western blot)
• Cell Tissue Type: Permanent cell lines

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