Anticorps Recombinant de lapin anti-IFITM3

IFITM3 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242056C9

N° de cat : 81135-3-RR

Synonymes

Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, IFITM2/3, DSPA2b, 242056C9

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Galerie de données de validation

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  WB analysis using 81135-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81135-3-RR (IFITM3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 81135-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IFITM3 antibody (81135-3-RR, Clone: 242056C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of MCF-7 using 81135-3-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug IFITM3 Recombinant antibody (81135-3-RR, Clone:242056C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 81135-3-RR

  Biolayer interferometry (BLl) kinetic assays of 81135-3-RR against Human IFITM3 were performed. The affinity constant is 0.911 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules THP-1
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81135-3-RR cible IFITM3 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFITM3 Protéine recombinante Ag2285
• Nom complet: interferon induced transmembrane protein 3 (1-8U)
• Masse moléculaire calculée: 133 aa, 15 kDa
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC006794
• Symbole du gène: IFITM3
• Identification du gène (NCBI): 10410
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression.

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