Anticorps Monoclonal anti-IFN alpha 2A

IFN alpha 2A Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

3C7G3

N° de cat : 68665-1-PBS

Synonymes

IFN alpha 2, IFNA2, IFN alpha2, IFN-alpha 2, IFN-alpha-2

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP50005-1

  Sandwich ELISA standard curve of MP50005-1, IFNA2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69008-1-PBS. Detection antibody: 68665-1-PBS. Standard: HZ-1066. Range: 7.8-500 pg/mL.

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  Sample test of MP50005-1

  Serum of six individual healthy human donors was measured. The IFN alpha 2 concentration of detected samples was determined to be 66.5 pg/mL with a range of 29.0-185.5 pg/mL.

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  Sample test of MP50005-1

  Human peripheral blood mononuclear cells (PBMC) was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were unstimulated or stimulated with 10 μg/mL poly I:C and Lipofectamine 2000 (LF2K) for 1 day. An aliquot of the cell culture supernatant was removed, the mean IFN alpha 2 concentration was undetectable in unstimulated PBMC supernatant, 159.7 pg/mL in poly I:C and LF2K stimulated PBMC supernatant.

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Informations sur le produit

68665-1-PBS cible IFN alpha 2A dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IFN alpha 2A Protéine recombinante HZ-1066
• Nom complet: interferon, alpha 2
• Symbole du gène: IFN alpha2
• Identification du gène (NCBI): 3440
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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