Anticorps Recombinant de lapin anti-IFNAR1

• Phare
• Validé par KD/KO

IFNAR1 Recombinant Antibody for WB, FC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

230549C8

N° de cat : 83002-4-RR

Synonymes

230549C8, AVP, CRF2 1, CRF2-1, Cytokine receptor class-II member 1

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Galerie de données de validation

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  WB analysis of HeLa using 83002-4-RR

  WB result of IFNAR1 antibody (83002-4-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFNAR1 transfected HeLa cells.

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  WB analysis using 83002-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83002-4-RR (IFNAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 83002-4-RR

  1x10^6 Jurkat cells were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

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  FC experiment of K562 using 83002-4-RR

  1x10^6 K562 were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 83002-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83002-4-RR against Human IFNAR1 were performed. The affinity constant is 2.04 nM.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en cytométrie: cellules Jurkat, K562

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83002-4-RR cible IFNAR1 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFNAR1 Protéine recombinante Eg0156
• Nom complet: interferon (alpha, beta and omega) receptor 1
• Masse moléculaire calculée: 557 aa, 64 kDa
• Poids moléculaire observé: 80-140 kDa
• Numéro d’acquisition GenBank: BC021825
• Symbole du gène: IFNAR1
• Identification du gène (NCBI): 3454
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.

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