Anticorps Polyclonal de lapin anti-IFN-gamma

• Phare
• Validé par KD/KO

IFN-gamma Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(132)

N° de cat : 15365-1-AP

Synonymes

IFNG, IFG, IFN gamma, IFN γ, IFNγ

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Galerie de données de validation

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  WB analysis using 15365-1-AP

  PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 15365-1-AP

  Recombinant human IFN gamma protein (cat.NO. HZ-1301) were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN gamma antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15365-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15365-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: NK-92 cells, Protéine recombinante
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 56 publications below
• IHC: See 46 publications below
• IF: See 35 publications below
• ELISA: See 1 publications below

Informations sur le produit

15365-1-AP cible IFN-gamma dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFN-gamma Protéine recombinante Ag8321
• Nom complet: IFN gamma
• Masse moléculaire calculée: 166 aa, 19 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC070256
• Symbole du gène: IFNG
• Identification du gène (NCBI): 3458
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Publications

Species	Application	Title
human	IF	Nat Immunol; Targeting regulator of G protein signaling 1 in tumor-specific T cells enhances their trafficking to breast cancer.; Authors - Di Huang
human	IF	Adv Sci (Weinh); Targeting Deltex E3 Ubiquitin Ligase 2 Inhibits Tumor-associated Neutrophils and Sensitizes Hepatocellular Carcinoma Cells to Immunotherapy; Authors - Xiaoling Wu
	IF	Acta Pharm Sin B; Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy; Authors - Hao Ren
human	IF	Gastroenterology; Interferon-γ-stimulated genes, but not USP18, are expressed in livers of patients with acute hepatitis C.; Authors - Michael T Dill
	WB,IHC	eBioMedicine; Histone deacetylases inhibitor chidamide synergizes with humanized PD1 antibody to enhance T-cell chemokine expression and augment Ifn-γ response in NK-T cell lymphoma; Authors - Tingyu Wen
	WB	J Control Release; Efficient gene delivery by multifunctional star poly (β-amino ester)s into difficult-to-transfect macrophages for M1 polarization; Authors - Tao Bo

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