Anticorps Monoclonal anti-IFNGR1

IFNGR1 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB,ELISA

Conjugaison

Non conjugué

CloneNo.

1H3A4

N° de cat : 66704-1-Ig

Synonymes

CD119, CDw119, FLJ45734, IFN gamma R1, IFN gamma receptor 1, IFNGR, IFNGR1, interferon gamma receptor 1

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Galerie de données de validation

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  WB analysis of HeLa using 66704-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66704-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 66704-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66704-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66704-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66704-1-Ig (IFNGR1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules T-47D

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66704-1-Ig cible IFNGR1 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IFNGR1 Protéine recombinante Ag27085
• Nom complet: interferon gamma receptor 1
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC005333
• Symbole du gène: IFNGR1
• Identification du gène (NCBI): 3459
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)