Anticorps Polyclonal de lapin anti-IFN-gamma R2

IFN-gamma R2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10266-1-PBS

Synonymes

IFNGR2, AF 1, AF-1, IFGR2, IFN gamma R2

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Galerie de données de validation

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  WB analysis using 10266-1-AP (same clone as 10266-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10266-1-AP (IFNGR2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10266-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 10266-1-AP (same clone as 10266-1-PBS)

  IP result of anti-IFNGR2 (IP:10266-1-AP, 4ug; Detection:10266-1-AP 1:500) with A549 cells lysate 1800ug. This data was developed using the same antibody clone with 10266-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10266-1-AP (same clone as 10266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10266-1-AP (IFNGR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10266-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10266-1-AP (same clone as 10266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10266-1-AP (IFNGR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10266-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10266-1-PBS cible IFN-gamma R2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFN-gamma R2 Protéine recombinante Ag0317
• Nom complet: interferon gamma receptor 2 (interferon gamma transducer 1)
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC003624
• Symbole du gène: IFNGR2
• Identification du gène (NCBI): 3460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit which attaches to IFN-γ with a significantly lower affinity. Two subunits bind one IFN-γ dimer. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

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