Anticorps Polyclonal de lapin anti-IFT140

• Phare
• Validé par KD/KO

IFT140 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(81)

Avis (1)

N° de cat : 17460-1-AP

Synonymes

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog

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Galerie de données de validation

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  WB analysis of HeLa using 17460-1-AP

  WB result of IFT140 antibody (17460-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFT140 transfected HeLa cells.

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  WB analysis using 17460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17460-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 17460-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17460-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of rat testis using 17460-1-AP

  IP result of anti-IFT140 (IP:17460-1-AP, 4ug; Detection:17460-1-AP 1:300) with rat testis tissue lysate 4400ug.

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  IHC staining of mouse testis using 17460-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 17460-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 17460-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse testis using 17460-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using IFT140 antibody (17460-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI (blue).

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  IF Staining of C2C12 using 17460-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT140 antibody (17460-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de rat
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 26 publications below
• IHC: See 4 publications below
• IF: See 63 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17460-1-AP cible IFT140 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFT140 Protéine recombinante Ag11119
• Nom complet: intraflagellar transport 140 homolog (Chlamydomonas)
• Masse moléculaire calculée: 1462 aa, 165 kDa
• Poids moléculaire observé: 150-165 kDa
• Numéro d’acquisition GenBank: BC035577
• Symbole du gène: IFT140
• Identification du gène (NCBI): 9742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

Publications

Species	Application	Title
human	IF	Nat Genet; Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.; Authors - Carol-Anne Martin
mouse	WB,IF	Cell; Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.; Authors - Siew Cheng Phua
		Hepatology; Biallelic mutations in TTC26 (IFT56) cause severe biliary ciliopathy in humans.; Authors - Ranad Shaheen
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	IF	Nat Commun; A distal centriolar protein network controls organelle maturation and asymmetry.; Authors - Lei Wang
mouse	WB	Nat Commun; Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling.; Authors - Linh T Vuong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-18-2024)

I tested the antibody in iF in LIGHT2 cells (commonly used to study Hh signalling). Unfortunately, I don't see the ciliary signal when overlapping IFT140 with ARL13B. This could be cell type specific or the conditions must be optimized to reveal it ? I used the standard iF protocol: PFA 4% 10 min cold methanol 5 min 1h 1% BSA in PBST 1/500 in blocking solution, O.N. at 4°C 5*5min PBST 1/500 in blocking solution, 1h at RT° 4*5min PBST + 5min PBS

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: LIGHT2

IFT140 Antibody Immunofluorescence validation (1/500 dilution) in LIGHT2 (Cat no:17460-1-AP)

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