Anticorps Recombinant de lapin anti-IFT140

IFT140 Recombinant Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

230231F7

N° de cat : 82947-1-RR

Synonymes

c305C8.4, c380F5.1, DKFZp564L232, FLJ10306, FLJ30571, gs114, IFT140, KIAA0590, WDTC2

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Galerie de données de validation

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  WB analysis of HeLa using 82947-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 82947-1-RR (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 82947-1-RR

  rat testis tissue were subjected to SDS PAGE followed by western blot with 82947-1-RR (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 82947-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-1-RR, Clone: 230231F7 ) at dilution of 1:120 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu testiculaire de rat
• Résultats positifs en IF/ICC: Starvation treated hTERT-RPE1 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82947-1-RR cible IFT140 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFT140 Protéine recombinante Ag11119
• Nom complet: intraflagellar transport 140 homolog (Chlamydomonas)
• Masse moléculaire calculée: 1462 aa, 165 kDa
• Poids moléculaire observé: 150-165 kDa
• Numéro d’acquisition GenBank: BC035577
• Symbole du gène: IFT140
• Identification du gène (NCBI): 9742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

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