Anticorps Recombinant de lapin anti-IFT140

IFT140 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230231D5

N° de cat : 82947-7-PBS

Synonymes

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5

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Galerie de données de validation

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  WB analysis of HeLa using 82947-7-RR (same clone as 82947-7-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 82947-7-RR (same clone as 82947-7-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 82947-7-RR (same clone as 82947-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-7-RR, Clone: 230231D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82947-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82947-7-PBS

  Biolayer interferometry (BLl) kinetic assays of 82947-7 -RR against Human IFT140 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82947-7-PBS cible IFT140 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFT140 Protéine recombinante Ag11119
• Nom complet: intraflagellar transport 140 homolog (Chlamydomonas)
• Masse moléculaire calculée: 1462 aa, 165 kDa
• Numéro d’acquisition GenBank: BC035577
• Symbole du gène: IFT140
• Identification du gène (NCBI): 9742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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