Anticorps Recombinant de lapin anti-IFT140

IFT140 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230231D5

N° de cat : 82947-7-RR

Synonymes

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog, 230231D5

---

Galerie de données de validation

• 

  WB analysis of HeLa using 82947-7-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 82947-7-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 82947-7-RR (IFT140 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of hTERT-RPE1 using 82947-7-RR

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT140 antibody (82947-7-RR, Clone: 230231D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

• 

  Affinity and Kinetic Characterization of 82947-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82947-7 -RR against Human IFT140 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IF/ICC: Starvation treated hTERT-RPE1 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82947-7-RR cible IFT140 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFT140 Protéine recombinante Ag11119
• Nom complet: intraflagellar transport 140 homolog (Chlamydomonas)
• Masse moléculaire calculée: 1462 aa, 165 kDa
• Poids moléculaire observé: 150-165 kDa
• Numéro d’acquisition GenBank: BC035577
• Symbole du gène: IFT140
• Identification du gène (NCBI): 9742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports (PMID: 20368623, 22282595).

{{ptg:RelatedPrimaryAntibodies}}