Anticorps Recombinant de lapin anti-IFT172

IFT172 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250711E6

N° de cat : 86109-1-PBS

Synonymes

IFT, KIAA1179, NPHP17, osm 1, SLB

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Galerie de données de validation

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  WB analysis of mouse testis using 86109-1-RR (same clone as 86109-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 86109-1-RR (IFT172 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86109-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 86109-1-RR (same clone as 86109-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 86109-1-RR (IFT172 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86109-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 86109-1-RR (same clone as 86109-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT172 antibody (86109-1-RR, Clone: 250711E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 86109-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86109-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86109-1-RR against Human IFT172 were performed. The affinity constant is 2.02 nM.

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Informations sur le produit

86109-1-PBS cible IFT172 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFT172 Protéine recombinante Ag28921
• Nom complet: intraflagellar transport 172 homolog (Chlamydomonas)
• Masse moléculaire calculée: 1749 aa, 198 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC137126
• Symbole du gène: IFT172
• Identification du gène (NCBI): 26160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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