Anticorps Polyclonal de lapin anti-IFT52

• Phare
• Validé par KD/KO

IFT52 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris et plus (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 17534-1-AP

Synonymes

C20orf9, CGI 53, IFT52, NGD5, Protein NGD5 homolog

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Galerie de données de validation

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  WB analysis using 17534-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17534-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 17534-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 17534-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17534-1-AP

  IP Result of anti-IFT52 (IP:17534-1-AP, 4ug; Detection:17534-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IP experiment of HeLa cells using 17534-1-AP

  IP result of anti-IFT52 (17534-1-AP for IP and Detection) with HeLa cell lysate.

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  IF Staining of HeLa using 17534-1-AP

  Immunofluorescent analysis of Hela cells, using IFT52 antibody 17534-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules LNCaP, cellules MDCK, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris, cellules HeLa
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17534-1-AP cible IFT52 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFT52 Protéine recombinante Ag11586
• Nom complet: intraflagellar transport 52 homolog (Chlamydomonas)
• Masse moléculaire calculée: 437 aa, 50 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC039831
• Symbole du gène: IFT52
• Identification du gène (NCBI): 51098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; The cilia protein IFT88 is required for spindle orientation in mitosis.; Authors - Delaval Benedicte B
human	WB	Nat Commun; IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.; Authors - Nicolas Taulet
human	WB	Am J Hum Genet; Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice.; Authors - Chunyu Liu
mouse	CoIP	Am J Hum Genet; Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.; Authors - Wangjie Liu
mouse	WB	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
Chicken	IP	Proc Natl Acad Sci U S A; FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.; Authors - Akira Honda