Anticorps Polyclonal de lapin anti-IFT81

• Phare
• Validé par KD/KO

IFT81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11744-1-PBS

Synonymes

Carnitine deficiency-associated protein expressed in ventricle 1, CDV 1, CDV 1R, CDV1, CDV-1

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Galerie de données de validation

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  WB analysis of mouse testis using 11744-1-AP (same clone as 11744-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  WB analysis using 11744-1-AP (same clone as 11744-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11744-1-AP (same clone as 11744-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11744-1-AP (same clone as 11744-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11744-1-AP (same clone as 11744-1-PBS)

  IP result of anti-IFT81 (IP:11744-1-AP, 3ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 7500ug. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11744-1-AP (same clone as 11744-1-PBS)

  IP result of anti-IFT81 (IP:11744-1-AP, 4ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 5200ug. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 11744-1-AP (same clone as 11744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11744-1-AP (IFT81 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 11744-1-AP (same clone as 11744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11744-1-AP (IFT81 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

  IF result (localization to the spindle poles) of anti-IFT81 (11744-1-AP, 1:50) with metaphase hTERT-RPE1 cells (MeOH fixed) byDr. Moshe Kim. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 11744-1-AP (same clone as 11744-1-PBS)

  Immunofluorescent images of MDCK cells stained with IFT81 rabbit pAb (11744-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for IFT81, and Rhodamine-Goat anti-rabbit IgG for 66200-1-Ig. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

  IF result (the base and tip of cilia) of anti-IFT81 (11744-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 11744-1-AP (same clone as 11744-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 11744-1-AP (same clone as 11744-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 11744-1-AP (same clone as 11744-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (11744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11744-1-PBS cible IFT81 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFT81 Protéine recombinante Ag2339
• Nom complet: intraflagellar transport 81 homolog (Chlamydomonas)
• Masse moléculaire calculée: 676 aa, 80 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC029349
• Symbole du gène: IFT81
• Identification du gène (NCBI): 28981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.

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