Anticorps Polyclonal de lapin anti-IFT88

• Phare
• Validé par KD/KO

IFT88 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

333

Avis (4)

N° de cat : 13967-1-AP

Synonymes

IFT88, TPR repeat protein 10, TTC10

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Galerie de données de validation

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  WB analysis using 13967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13967-1-AP (IFT88 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13967-1-AP (IFT88 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of knockout cells and WT cells using 13967-1-AP

  IP and WB result of IFT88 (13967-1-AP) from Dr. Corbit, Kevin. Knockout cells and WT cells.

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  IP experiment of HEK-293 using 13967-1-AP

  IP Result of anti-IFT88 (IP:13967-1-AP, 3ug; Detection:13967-1-AP 1:800) with HEK-293 cells lysate 1500ug.

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  IHC staining of human heart using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13967-1-AP (IFT88 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13967-1-AP (IFT88 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13967-1-AP (IFT88 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13967-1-AP (IFT88 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of MDCK using 13967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 13967-1-AP (IFT88 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of hTERT-RPE1 using 13967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT88 antibody (13967-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 13967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT88 antibody (13967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MDCK using 13967-1-AP

  Immunofluorescent images of MDCK cells stained with IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated AffiniPure Goat anti-Mouse IgG(H+L) for 66200-1-Ig.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules MDCK, cellules NIH/3T3, tissu de thymus de souris
• Résultats positifs en IP: cellules knockout et cellules WT, cellules HEK-293
• Résultats positifs en IHC: tissu cardiaque humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MDCK, cellules C2C12, cellules hTERT-RPE1

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 48 publications below
• WB: See 139 publications below
• IHC: See 5 publications below
• IF: See 231 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

13967-1-AP cible IFT88 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, canin, Humain, poisson-zèbre, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFT88 Protéine recombinante Ag4980
• Nom complet: intraflagellar transport 88 homolog (Chlamydomonas)
• Masse moléculaire calculée: 94 kDa
• Poids moléculaire observé: 88-95 kDa
• Numéro d’acquisition GenBank: BC030776
• Symbole du gène: IFT88
• Identification du gène (NCBI): 8100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

Publications

Species	Application	Title
mouse	IF	Nature; Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein.; Authors - Firestone Ari J AJ
mouse	WB	Nature; Functional interaction between autophagy and ciliogenesis.; Authors - Pampliega Olatz O
chicken	IF	Science; Apical abscission alters cell polarity and dismantles the primary cilium during neurogenesis.; Authors - Raman M Das
mouse	IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	IF	Nat Genet; Complex interactions between genes controlling trafficking in primary cilia.; Authors - Ocbina Polloneal Jymmiel R PJ
human	IF	Nat Genet; Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.; Authors - Carol-Anne Martin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Serhiy (Verified Customer) (01-04-2023)

This antibody detects Ift88 in ciliary axonemes of immunofluorescently stained IMCD3 cells (atteched image: red Ift88; green - gamma-tubulin) and mouse fibroblasts. We also tried to detect endogenous Ift88 in NIH3T3 cells by western blots and found that antibody detects several non-specific bands in addition to apparently specific one between the 75-100 kDa markers.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100 (IF), 1:1000 (WB)
• Cell Tissue Type: NIH3T3 fibroblasts, IMCD3 kidney collecting duct cells

IFT88 Antibody Immunofluorescence validation (1:100 (IF), 1:1000 (WB) dilution) in NIH3T3 fibroblasts, IMCD3 kidney collecting duct cells (Cat no:13967-1-AP)

FH

Charlotte (Verified Customer) (07-26-2022)

We have revealed IFT88 after stripping a couple of times. The signal remains nice and the unspecific bands might be due to lower stripping efficiency

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: NIH-3T3

IFT88 Antibody Western Blot,Immunofluorescence validation (1/500 dilution) in NIH-3T3 (Cat no:13967-1-AP)

FH

Stephen (Verified Customer) (02-08-2022)

works well in RPE cells stains Primary cilia seen by co-staining with Ac-Tubulin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human RPE-1 cells

IFT88 Antibody Immunofluorescence validation (1:500 dilution) in Human RPE-1 cells (Cat no:13967-1-AP)

FH

Boyan (Verified Customer) (01-31-2019)

Excellent. Works quite well both in WB and IF.

• Applications: Western Blot, Immunofluorescence,

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