Anticorps Monoclonal anti-IFT88

• Phare
• Validé par KD/KO

IFT88 Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4A4G5

N° de cat : 60227-1-PBS

Synonymes

4A4G5, Intraflagellar transport protein 88 homolog, TG737, TTC10

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Galerie de données de validation

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  WB analysis of human testis using 60227-1-Ig (same clone as 60227-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 60227-1-Ig (same clone as 60227-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 60227-1-Ig (same clone as 60227-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 60227-1-Ig (same clone as 60227-1-PBS)

  IP result of anti-IFT88 (IP:60227-1-Ig, 5ug; Detection:60227-1-Ig 1:800) with PC-3 cells lysate 1040ug. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 60227-1-Ig (same clone as 60227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60227-1-PBS cible IFT88 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IFT88 Protéine recombinante Ag4980
• Nom complet: intraflagellar transport 88 homolog (Chlamydomonas)
• Masse moléculaire calculée: 94 kDa
• Poids moléculaire observé: 88-95 kDa
• Numéro d’acquisition GenBank: BC030776
• Symbole du gène: IFT88
• Identification du gène (NCBI): 8100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

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