Anticorps Recombinant de lapin anti-IGF1

IGF1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241890G6

N° de cat : 84782-5-RR

Synonymes

241890G6, Igf-1, IGF-I, Insulin-like growth factor I, Somatomedin

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Galerie de données de validation

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  WB analysis of rat plasma using 84782-5-RR

  rat plasma were subjected to SDS PAGE followed by western blot with 84782-5-RR (IGF-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 84782-5-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84782-5-RR (IGF1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 84782-5-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84782-5-RR (IGF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Transfected HEK-293T using 84782-5-RR

  1x10^6 HEK-293T cells or IGF1 Transfected HEK-293T cells were intracellularly stained with 0.25 ug IGF1 Recombinant antibody (84782-5-RR, Clone:241890G6) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84782-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84782-5-RR against Rat IGF1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: rat plasma,
• Résultats positifs en IHC: tissu hépatique de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84782-5-RR cible IGF1 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IGF1 Protéine recombinante Eg1976
• Nom complet: insulin-like growth factor 1
• Masse moléculaire calculée: 14kd
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: AAA41386
• Symbole du gène: Igf1
• Identification du gène (NCBI): 24482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGF1, also named as IBP1, MGF, IGF-IA, and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but has a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

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