Anticorps Polyclonal de lapin anti-IGF1A-Specific

• Phare
• Validé par KD/KO

IGF1A-Specific Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 20214-1-AP

Synonymes

IBP1, IGF I, IGF1, IGF-1, IGF1A, IGF1A-Specific, IGF1B, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C

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Galerie de données de validation

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  IHC staining of mouse liver using 20214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20214-1-AP (IGF1A-Specific antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20214-1-AP (IGF1A-Specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20214-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20214-1-AP (IGF1A-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20214-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20214-1-AP (IGF1A-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu hépatique de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

20214-1-AP cible IGF1A-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: insulin-like growth factor 1 (somatomedin C)
• Masse moléculaire calculée: 17 kDa
• Symbole du gène: IGF1
• Identification du gène (NCBI): 3479
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGF1, also named as IBP1, MGF, IGF-IA and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1A.

Publications

Species	Application	Title
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
mouse	WB	J Hepatol; Deficiency of insulin-like growth factor 1 attenuates aging-induced changes in hepatic function: Role of autophagy.; Authors - Xu Xihui X
mouse	WB,IF	Proc Natl Acad Sci U S A; Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.; Authors - Liang Chen
mouse	WB,IHC	Int J Cancer; Estrogen and insulin-like growth factor synergistically promote the development of lung adenocarcinoma in mice.; Authors - Tang Hexiao H
mouse	WB	Mol Neurobiol; Intramuscular Delivery of scAAV9-hIGF1 Prolongs Survival in the hSOD1(G93A) ALS Mouse Model via Upregulation of D-Amino Acid Oxidase.; Authors - HuiQian Lin
human	WB	Cancer Lett; IFN-γ-mediated IRF1/miR-29b feedback loop suppresses colorectal cancer cell growth and metastasis by repressing IGF1.; Authors - Li Yuan

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