Anticorps Recombinant de lapin anti-IGF1R/CD221

IGF1R/CD221 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241678B8

N° de cat : 84385-3-RR

Synonymes

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

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Galerie de données de validation

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  WB analysis using 84385-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84385-3-RR (IGF1R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 84385-3-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IGF1R antibody (84385-3-RR, Clone: 241678B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84385-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84385-3-RR against Human IGF1R were performed. The affinity constant is 0.239 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules L02
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84385-3-RR cible IGF1R/CD221 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: IGF I receptor
• Masse moléculaire calculée: 1367 aa, 155 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC113612
• Symbole du gène: IGF1R
• Identification du gène (NCBI): 3480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.

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