Anticorps Recombinant de lapin anti-IGF2

IGF2 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

241746D3

N° de cat : 84643-1-RR

Synonymes

241746D3, IGF II, IGF-II, Insulin-like growth factor II Ala-25 Del, PP1446

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Galerie de données de validation

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  WB analysis of HuH-7 using 84643-1-RR

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 84643-1-RR (IGF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 84643-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84643-1-RR (IGF2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84643-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84643-1-RR (IGF2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84643-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84643-1-RR against Human IGF2 were performed. The affinity constant is 0.159 nM.

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Applications testées

• Résultats positifs en WB: cellules HuH-7,
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84643-1-RR cible IGF2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IGF2 Protéine recombinante HZ-1161
• Nom complet: insulin-like growth factor 2 (somatomedin A)
• Poids moléculaire observé: 20 kDa
• Symbole du gène: IGF2
• Identification du gène (NCBI): 3481
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-like growth factor 2 (IGF2) is a crucial protein that plays a significant role in regulating growth, particularly during normal fetal development, and is also often dysregulated during tumorigenesis. IGF2 is one of the main ligands of the type 1 IGF receptor (IGF-1R), a tyrosine kinase receptor that transmits signals upon binding with IGF-II. IGF2 is predominantly secreted via the placenta during pregnancy and, after birth, primarily by hepatocytes, with its secretion being independent of growth hormone (GH) secretion, unlike IGF-I.

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