Anticorps Recombinant de lapin anti-IGF2BP2

IGF2BP2 Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6L8

N° de cat : 82757-2-PBS

Synonymes

insulin like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2

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Galerie de données de validation

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  WB analysis using 82757-2-RR (same clone as 82757-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82757-2-RR (IGF2BP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 82757-2-RR (same clone as 82757-2-PBS)

  IP result of anti-IGF2BP2 (IP:82757-2-RR, 4ug; Detection:82757-2-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 82757-2-RR (same clone as 82757-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 82757-2-RR (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82757-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82757-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82757-2-RR against Human IGF2BP2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82757-2-PBS cible IGF2BP2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IGF2BP2 Protéine recombinante Ag2142
• Nom complet: insulin-like growth factor 2 mRNA binding protein 2
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC021290
• Symbole du gène: IGF2BP2
• Identification du gène (NCBI): 10644
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).

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