Anticorps Recombinant de lapin anti-IGF2BP3

• Phare
• Validé par KD/KO

IGF2BP3 Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1L11

N° de cat : 81805-1-PBS

Synonymes

1L11, IGF2 mRNA-binding protein 3, IGF2BP 3, IGF-II mRNA-binding protein 3, IMP 3

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Galerie de données de validation

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  WB analysis using 81805-1-RR (same clone as 81805-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81805-1-RR (IGF2BP3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81805-1-RR (same clone as 81805-1-PBS)

  WB result of IGF2BP3 antibody (81805-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IGF2BP3 transfected HeLa cells. This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 81805-1-RR (same clone as 81805-1-PBS)

  IP result of anti-IGF2BP3 (IP:81805-1-RR, 4ug; Detection:81805-1-RR 1:20000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 81805-1-RR (same clone as 81805-1-PBS)

  IP result of anti-IGF2BP3 (IP:81805-1-RR, 4ug; Detection:81805-1-RR 1:4000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical squamous cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81805-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81805-1-RR against Human IGF2BP3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

81805-1-PBS cible IGF2BP3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IGF2BP3 Protéine recombinante Ag6226
• Nom complet: insulin-like growth factor 2 mRNA binding protein 3
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC065269
• Symbole du gène: IGF2BP3
• Identification du gène (NCBI): 10643
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

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