Anticorps Polyclonal de lapin anti-IGFBP1

IGFBP1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13981-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 13981-1-AP (same clone as 13981-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13981-1-AP (IGFBP1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13981-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 13981-1-AP (same clone as 13981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13981-1-AP (IGFBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13981-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

13981-1-PBS cible IGFBP1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IGFBP1 Protéine recombinante Ag5026
• Nom complet: IGF binding protein 1
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-35 kDa
• Numéro d’acquisition GenBank: BC057806
• Symbole du gène: IGFBP1
• Identification du gène (NCBI): 3484
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IGF-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and INS resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.

{{ptg:RelatedPrimaryAntibodies}}