Anticorps Polyclonal de lapin anti-IGFBP3

• Phare
• Validé par KD/KO

IGFBP3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10189-2-PBS

Synonymes

BP 53, IBP 3, IBP3, IBP-3, IGF binding protein 3

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Galerie de données de validation

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  WB analysis using 10189-2-AP (same clone as 10189-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10189-2-AP (same clone as 10189-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10189-2-AP (same clone as 10189-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10189-2-AP (same clone as 10189-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10189-2-AP (same clone as 10189-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10189-2-AP (same clone as 10189-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP3 antibody (10189-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10189-2-AP (same clone as 10189-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP3 antibody (10189-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

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Informations sur le produit

10189-2-PBS cible IGFBP3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IGFBP3 Protéine recombinante Ag0242
• Nom complet: insulin-like growth factor binding protein 3
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa, 40-45 kDa
• Numéro d’acquisition GenBank: BC000013
• Symbole du gène: IGFBP3
• Identification du gène (NCBI): 3486
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)

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