Anticorps Monoclonal anti-IGFBP3

IGFBP3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A12A11

N° de cat : 68402-1-PBS

Synonymes

IGF binding protein 3, IBP-3, IBP3, IBP 3, BP 53

---

Galerie de données de validation

• 

  WB analysis of human placenta using 68402-1-Ig (same clone as 68402-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68402-1-Ig (IGFBP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68402-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68402-1-Ig (same clone as 68402-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68402-1-Ig (IGFBP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 68402-1-Ig (same clone as 68402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68402-1-Ig (IGFBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68402-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68402-1-PBS cible IGFBP3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IGFBP3 Protéine recombinante Ag20351
• Nom complet: insulin-like growth factor binding protein 3
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 39-45 kDa
• Numéro d’acquisition GenBank: BC000013
• Symbole du gène: IGFBP3
• Identification du gène (NCBI): 3486
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)

{{ptg:RelatedPrimaryAntibodies}}