Anticorps Recombinant de lapin anti-IGFBP4

IGFBP4 Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230543F8

N° de cat : 83200-3-PBS

Synonymes

IBP 4, HT29 IGFBP, Deb2, BP4, BP 4

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Galerie de données de validation

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  WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

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  WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83200-3-RR (same clone as 83200-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83200-3-RR (IGFBP4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83200-3-RR (same clone as 83200-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug IGFBP4 Recombinant antibody (83200-3-RR, Clone:230543F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83200-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83200-3-RR against Human IGFBP4 were performed. The affinity constant is 0.776 nM.

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Informations sur le produit

83200-3-PBS cible IGFBP4 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: insulin-like growth factor binding protein 4
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-40 kDa
• Numéro d’acquisition GenBank: NM_001552.3
• Symbole du gène: IGFBP4
• Identification du gène (NCBI): 3487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401).

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