Anticorps Polyclonal de lapin anti-IGFBP5

IGFBP5 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 55205-1-AP

Synonymes

IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5

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Galerie de données de validation

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  WB analysis of SKOV-3 using 55205-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 55205-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 55205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55205-1-AP (IGFBP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55205-1-AP (IGFBP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 55205-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using IGFBP5 antibody (55205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, tissu embryonnaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

55205-1-AP cible IGFBP5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: insulin-like growth factor binding protein 5
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: NM_000599
• Symbole du gène: IGFBP5
• Identification du gène (NCBI): 3488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.

Publications

Species	Application	Title
human	IF	Theranostics; Material-induced Senescence (MIS): Fluidity Induces Senescent Type Cell Death of Lung Cancer Cells via Insulin-Like Growth Factor Binding Protein 5.; Authors - Sharmy Saimon Mano
human	WB	Oncogene; Fibulin-3 promotes muscle-invasive bladder cancer.; Authors - A L Han
human	IF	Breast Cancer Res; Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors; Authors - Wei Yang
mouse	WB	Transl Res; PMID: 30392876; Authors - Lin Xihua
human	WB	J Proteomics; Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.; Authors - Sun-Xia Chen
rat	WB	Am J Physiol Heart Circ Physiol; Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.; Authors - Guangrui Lai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Margarita (Verified Customer) (06-06-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: CWR22Rv1 cells

IGFBP5 Antibody Immunofluorescence validation (1:100 dilution) in CWR22Rv1 cells (Cat no:55205-1-AP)

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