Anticorps Monoclonal anti-IGFBP6

IGFBP6 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1D12B3

N° de cat : 67567-1-Ig

Synonymes

IBP 6, IBP6, IGF binding protein 6, IGFBP 6, IGFBP6

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Galerie de données de validation

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  WB analysis of U-87 MG using 67567-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67567-1-Ig (IGFBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 67567-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67567-1-Ig (IGFBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 67567-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67567-1-Ig (IGFBP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67567-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67567-1-Ig (IGFBP6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MG U-87, tissu testiculaire humain
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

67567-1-Ig cible IGFBP6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, singe
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IGFBP6 Protéine recombinante Ag30050
• Nom complet: insulin-like growth factor binding protein 6
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC011708
• Symbole du gène: IGFBP6
• Identification du gène (NCBI): 3489
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-like growth factor (IGF) binding protein (IGFBP6), a 240 amino acid protein, contains an IGFBP N-terminal domain and a thyroglobulin type-1 domain. It modulates the activity of IGF and shows independent effects of IGF, such as growth inhibition and apoptosis. It can decrease the proliferation and survival of cancer cells such as lung cancer cells and naso-pharyngeal cancer cells. IGFBP-6 is distinctive for its 50-fold higher binding affinity for IGF-II over IGF-I and this specificity makes it an attractive potential therapeutic candidate for IGF-II-dependent pediatric malignancies such as rhabdomyosarcoma (RMS). In addition, it was found that IGFBP6 can promote the migration of RMS cells in an IGF-independent manner, and MAPK pathways were involved in this process. Further study reported that IGFBP6 is one of most highly expressed proteins in varicose vein tissues and is involved in the proliferation of vascular smooth muscle cells (VSMCs), which may provide insights into the underlying pathogenesis of varicose vein.

Publications

Species	Application	Title
human	IHC	Neurol Res; The clinical characteristics and prognostic value of IGFBP6 in glioma.; Authors - Zhitao Zong
monkey	IHC	Front Cell Dev Biol; Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone; Authors - Hiroki Hagizawa